N-bromosuccinmide in tetrahydrofuran; methanol T=0°C; 3.16667 h;

Ascophyllum nodosum vanadate(V)-dependent bromoperoxidase I; dihydrogen peroxide; sodium bromide in tert-butyl alcohol T=23°C; pH=6.2; 24 h; aq. bufferEnzymatic reaction;

2-(n-morpholino)ethanesulfonic acid; VBrPO(AnI) bromoperoxidase; dihydrogen peroxide; sodium bromide in water; tert-butyl alcohol T=20°C; pH=6.2; ConcentrationReagent/catalyst;

1: sodium methylate / 0.5 h / 23 °C / Inert atmosphere 2: Ascophyllum nodosum vanadate(V)-dependent bromoperoxidase I; dihydrogen peroxide; sodium bromide / tert-butyl alcohol / 24 h / 23 °C / pH 6.2 / aq. buffer; Enzymatic reaction View Scheme

1: N-bromosuccinimide / tetrahydrofuran / -78 °C 2: 1.) lithium diisopropylamide / 1.) THF, hexane, a.) -78 deg C, b.) -40 deg C, 2.) THF, -78 deg C, 1 h 3: 5percent sodium hydroxide / methanol / Ambient temperature View Scheme

1: methanol / 0.67 h / 23 °C / Inert atmosphere 2: Ascophyllum nodosum vanadate(V)-dependent bromoperoxidase I; dihydrogen peroxide; sodium bromide / water; tert-butyl alcohol / 24 h / 23 °C / pH 6.2 / aq. buffer; Enzymatic reaction View Scheme