sodium hydroxide; subtilisin in phosphate buffer T=20°C; 4 h;

<b>Multi-step reaction with 4 steps<br /> </b>1: 91 percent / thionyl chloride / -10 - 20 &deg;C<br />2: 98 percent / sodium carbonate / dioxane; H₂O / 0 - 20 &deg;C<br />3: 83 percent / triphenylphosphine; N-bromo-succinimide / tetrahydrofuran / 0 - 20 &deg;C<br />4: 100 percent / sodium sulfite; H₂O / dioxane / 1 h / 100 &deg;C<br /> <br /> &nbsp;

<b>Multi-step reaction with 3 steps<br /> </b>1: 98 percent / sodium carbonate / dioxane; H₂O / 0 - 20 &deg;C<br />2: 83 percent / triphenylphosphine; N-bromo-succinimide / tetrahydrofuran / 0 - 20 &deg;C<br />3: 100 percent / sodium sulfite; H₂O / dioxane / 1 h / 100 &deg;C<br /> <br /> &nbsp;