HEPES buffer; recombinant alanine racemase alrY265A in water T=30°C; pH=8; Enzyme kinetics;

<b>Multi-step reaction with 5 steps<br /> </b>1: AlCl₂Et / diethyl ether; hexane / -78 &deg;C<br />2: 85 percent / NaH / dimethylformamide / 5 h / 0 &deg;C<br />3: 100 percent / 6M aq. HCl / tetrahydrofuran / 2 h / 20 &deg;C<br />4: Jones reagent / acetone / 5 h / 0 - 20 &deg;C<br />5: 6M aq. HCl / ethyl acetate / 12 h / 100 &deg;C<br /> <br /> &nbsp;

<b>Multi-step reaction with 3 steps<br /> </b>1: 64 percent / LDA / tetrahydrofuran / 1 h / -100 &deg;C<br />3: 62 percent / Raney-Ni / H₂O / 0.25 h / 70 &deg;C<br /> <br /> &nbsp;