1: NaOH 2: 68 percent / tetrahydrofuran / 0.25 h / 0 °C 3: plasmid-containing strain of E coli BN 116, NH4(1+) View Scheme

<b>Multi-step reaction with 3 steps<br /> </b>1: 94 percent / Et₃N / CH₂Cl₂ / Ambient temperature<br />2: CH₂Cl₂ / Ambient temperature<br />3: 47 percent / NaOH / tetrahydrofuran; H₂O<br /> <br /> &nbsp;