sulfuric acid in water T=5°C; pH=1 - 2;

1: phenol / 4.5 h / 50 °C 2: penicillin G acylase / water / 37 °C / pH 8 - 8.5 / aq. acetate buffer; Enzymatic reaction 3: tetrahydrofuran; water / 6.5 h / 25 °C / pH 6.8 - 7.2 4: sulfuric acid / water / 5 °C / pH 1 - 2 View Scheme

1: penicillin G acylase / water / 37 °C / pH 8 - 8.5 / aq. acetate buffer; Enzymatic reaction 2: tetrahydrofuran; water / 6.5 h / 25 °C / pH 6.8 - 7.2 3: sulfuric acid / water / 5 °C / pH 1 - 2 View Scheme

1: sodium iodide / acetone / 5 h / -5 - 0 °C / Darkness; Inert atmosphere 2: ethyl acetate / -5 °C / Inert atmosphere 3: phenol / 4.5 h / 50 °C 4: penicillin G acylase / water / 37 °C / pH 8 - 8.5 / aq. acetate buffer; Enzymatic reaction 5: tetrahydrofuran; water / 6.5 h / 25 °C / pH 6.8 - 7.2 6: sulfuric acid / water / 5 °C / pH 1 - 2 View Scheme

1: ethyl acetate / -5 °C / Inert atmosphere 2: phenol / 4.5 h / 50 °C 3: penicillin G acylase / water / 37 °C / pH 8 - 8.5 / aq. acetate buffer; Enzymatic reaction 4: tetrahydrofuran; water / 6.5 h / 25 °C / pH 6.8 - 7.2 5: sulfuric acid / water / 5 °C / pH 1 - 2 View Scheme