1: 81 percent / O2 / D-amino acid oxidase (immobilized on glyoxyl agarose) / aq. phosphate buffer / 3.33 h / 25 °C / pH 7.5 / Enzymatic reaction 2: aq. NaOH / glutaryl acylase (immobilized on polyethyleneimine) / aq. phosphate buffer / 0.5 h / 25 °C / pH 8.0 / Enzymatic reaction View Scheme

sodium hydroxide; D-amino acid oxidase; bovine catalase (on glyoxyl agarose); glutaryl acylase (immobilized on polyethyleneimine) in phosphate buffer T=25°C; pH=8; 3 h; Enzymatic reaction;

phosphate buffer; enzyme derivative DAO; enzyme derivative GA T=25°C; 2 h; pH 8;

sodium hydroxide; D-amino acid oxidase; bovine catalase (on glyoxyl agarose); glutaryl acylase (immobilized on polyethyleneimine) in phosphate buffer T=25°C; pH=8; 3 h; Enzymatic reaction;

1: 10.3 percent / O2 / D-amino acid oxidase (immobilized on glyoxyl agarose) / aq. phosphate buffer / 3.33 h / 25 °C / pH 7.5 / Enzymatic reaction 2: aq. NaOH / glutaryl acylase (immobilized on polyethyleneimine) / aq. phosphate buffer / 0.5 h / 25 °C / pH 8.0 / Enzymatic reaction View Scheme

1: 96 percent / D-aminoacid oxidase (DAO), glutaryl acylase (GA) / H2O / 2.5 h / pH = 8, O2 2: 98 percent / penicillin G acylase (PGA) / H2O / 4 °C / pH = 6.5 3: 70 percent / H2O / 65 °C View Scheme