phosphate buffer; 1,4-dithio-DL-erythritol; pyrimidine deaminase; riboflavin synthase; magnesium chloride; alkaline phosphatase 1.) 1 h, 37 deg C, 2.) 3 h, 37 deg C; Yield given. Multistep reaction;

1) cell extract of Candida guilliermondii ATCC 9058, phosphatase; 2) riboflavin synthase; Yield given. Multistep reaction;